Enterovirus survey in children after mass vaccination with live attenuated polioviruses.

Evidence for nonpoliovirus enterovirus multiplication in L20B cells.

Stool specimens collected from 1 515 healthy children following a mass vaccination campaign in Cuba were tested for poliovirus excretion using L20B cell lines. In spite of the selectivity of this cell line for polioviruses (117/129; 90.7%) some other nonpolio enteroviruses (12/129: 9.3%), such as coxsackie A virus types 4, 8 and 10, can grow in L20B cells.

Mass immunization of children in Iran with live attenuated Sugiyama measles virus adapted to calf kidney cell cultures

Assessment of cell culture and polymerase chain reaction procedures for the detection of polioviruses in wastewater.

WHO considers that environmental surveillance for wild-type polioviruses is potentially important for surveillance for acute flaccid paralysis as a means of confirming eradication of poliomyelitis. The present study investigated methods for detecting polioviruses in a variety of water environments in South Africa. Most polioviruses were isolated on L20B mouse cells, which, however, were not sel...

Use of monoclonal antibodies to identify serotypes of enterovirus isolates.

Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of enteroviruses is cell culture isolation, followed by serotype identification by neutralization assay. These procedures are time-consuming and expensive. Rapid serotype identification of enteroviruses is important in differentiating nonpoliovirus e...

Efficacy and safety of live attenuated Sugiyama strain of measles virus in mass immunization of children in rural regions of Iran